Difference between revisions of "Part:BBa K1072010"
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<p>Figure 1. a diagram of Odr-10, 7-transmembrane receptors.</p>
 
<p>Figure 1. a diagram of Odr-10, 7-transmembrane receptors.</p>
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We fuse it with plasma membrane protein,Odr-10 and expressed in yeast successfully figure 1.
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<p>Figure 1. fluorescence was detected around plasma membrane</p>
 
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Revision as of 08:43, 29 September 2013

Odr-10(diacetyl)GPCR with optimized codon

Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.

If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein. pathway is composed of Gpa1 (Gα), Ste4 (Gβ) and Ste18 (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ.

Figure 1. a diagram of Odr-10, 7-transmembrane receptors.


We fuse it with plasma membrane protein,Odr-10 and expressed in yeast successfully figure 1.


Figure 1. fluorescence was detected around plasma membrane


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]