Difference between revisions of "Part:BBa K1051304"

Revision as of 13:57, 28 September 2013

cln3 promoter (M phase to G1 phase)

Purpose

work to activates Cdc28p kinase to promote the G1 to S phase transition

Principle

CLn3 is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .it plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetly-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry.

We also captured fluorescence on cln3 promoter (S phase) test device, for the fluorescence is quite weak, we utilize micro plate reader and flow cytometry to verify the promoter.

Figure. Flow cytometry to verify S phase promoter cln3.

Sequence and Features