Difference between revisions of "Part:BBa K1150006"
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Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therfore they need receptors to detect different light wavelengts. Ultraviolet B (UVB) light can be perceived with photoreceptor UV resistance Locus 8 (UVR8) [1]. This UVR8 receptor derives drom ''Arapidopsis thaliana'' [2].
 
Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therfore they need receptors to detect different light wavelengts. Ultraviolet B (UVB) light can be perceived with photoreceptor UV resistance Locus 8 (UVR8) [1]. This UVR8 receptor derives drom ''Arapidopsis thaliana'' [2].
  
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==Usage and Biology==
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==Biology and Usage==
  
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In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVR8 monomerizes and changes into its open configuration. UVR8 is then able to recruit [https://parts.igem.org/Part:BBa_K1150007 COP1] [2]. This interaction is disrupted when UVB radiation stops. Plants use this mechanism to perceive UVB light and respond with photomorphogenesis or react to UVB induced stress [3]
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In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVR8 monomerizes and changes into its open configuration. UVR8 is then able to recruit [https://parts.igem.org/Part:BBa_K1150007 COP1] [2]. This interaction is disrupted when UVB radiation stops. Plants use this mechanism to perceive UVB light and respond with photomorphogenesis or react to UVB induced stress [3].
  
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Freiburg 2013 used this part to engineer a UVB light induced mechanism to activate gene expression. You need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 Cas9-UVR8], [https://parts.igem.org/Part:BBa_K1150030 COP1-VP16], [https://parts.igem.org/Part:BBa_K1150034 RNaimer]
  
 
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[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332
 
[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332
 +
 
[2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12
 
[2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12
 +
 
[3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600
 
[3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600
  

Revision as of 13:06, 28 September 2013

UVR8

dCas9
Function UVB receptor
Use in Mammalians and plants
RFC standard RFC 25
Backbone pSB1C3
Organism Arabidopsis thaliana
Source Konrad Müller
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therfore they need receptors to detect different light wavelengts. Ultraviolet B (UVB) light can be perceived with photoreceptor UV resistance Locus 8 (UVR8) [1]. This UVR8 receptor derives drom Arapidopsis thaliana [2].

Biology and Usage

In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVR8 monomerizes and changes into its open configuration. UVR8 is then able to recruit COP1 [2]. This interaction is disrupted when UVB radiation stops. Plants use this mechanism to perceive UVB light and respond with photomorphogenesis or react to UVB induced stress [3].

Freiburg 2013 used this part to engineer a UVB light induced mechanism to activate gene expression. You need a combination of these BioBricks: Cas9-UVR8, COP1-VP16, RNaimer

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 52

Literature

[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332

[2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12

[3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600