Difference between revisions of "Part:BBa K1150006"
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==Usage and Biology== | ==Usage and Biology== | ||
| − | In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived | + | In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVR8 monomerizes and changes into its open configuration. UVR8 is then able to recruit [https://parts.igem.org/Part:BBa_K1150007 COP1] [2]. This interaction is disrupted when UVB radiation stops. Plants use this mechanism to perceive UVB light and respond with photomorphogenesis or react to UVB induced stress [3] |
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<span class='h3bb'> | <span class='h3bb'> | ||
==Sequence and Features== | ==Sequence and Features== | ||
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==Literature== | ==Literature== | ||
<small> | <small> | ||
| − | [1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein | + | [1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332 |
| + | [2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12 | ||
| + | [3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600 | ||
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Revision as of 12:49, 28 September 2013
UVR8
| dCas9 | |
|---|---|
| Function | UVB receptor |
| Use in | Mammalians and plants |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Organism | Arabidopsis thaliana |
| Source | Konrad Müller |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therfore they need receptors to detect different light wavelengts. Ultraviolet B (UVB) light can be perceived with photoreceptor UV resistance Locus 8 (UVR8) [1]. This UVR8 receptor derives drom Arapidopsis thaliana [2].
Usage and Biology
In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVR8 monomerizes and changes into its open configuration. UVR8 is then able to recruit COP1 [2]. This interaction is disrupted when UVB radiation stops. Plants use this mechanism to perceive UVB light and respond with photomorphogenesis or react to UVB induced stress [3]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 37
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 52
Literature
[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332 [2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12 [3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600