Difference between revisions of "Part:BBa K1073022:Experience"

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According to these results the optical density for growth curves of cells expressing this protein was measured at 520 nm with respect to the chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>) and aeBlue (<partinfo>BBa_K1073020</partinfo>) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.  
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According to these results the optical density for growth curves of cells expressing this protein was determined at 520 nm with respect to the chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>) and aeBlue (<partinfo>BBa_K1073020</partinfo>) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.  
  
 
[[File:Fluorescence.JPG |200px]]
 
[[File:Fluorescence.JPG |200px]]

Revision as of 11:40, 28 September 2013


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Please enter how you used this part and how it worked out.

Applications of BBa_K1073022

When eforRed is expressed, the colonies on agar plates as well as liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to distinguish two different strains when cultivation in a mixed culture.

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iGEM Team Braunschweig 2013

When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined to be at 520nm.

BSAbsorption-eforRed.jpg

iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm.

Absorption maximum of eforRed: 534 nm

BS2013Emission eforRed.jpg

iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. This chromoprotein does show significant emission and can be detected by its fluorescence.

Emission maximum of eforRed: 605,4 nm


According to these results the optical density for growth curves of cells expressing this protein was determined at 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.

Fluorescence.JPG

iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.

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