Difference between revisions of "Part:BBa K1073022:Experience"

iGEM Team Braunschweig 2013

When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined to be at 520nm.

iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm.

Absorption maximum of eforRed: 534 nm

iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. This chromoprotein does show significant emission and can be detected by its fluorescence.

Emission maximum of eforRed: 605,4 nm

According to these results the optical density for growth curves of cells expressing this protein was measured at 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.

iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.