Difference between revisions of "Part:BBa K1189024"

Revision as of 11:11, 28 September 2013

Light chain human ferritin

This light ferritin chain comes from humans. This part along with heavy ferritin (BBa_K1189025), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is a robust, remaining stable at extreme pHs and temperatures. The light ferritin purportedly contributes to nucleation to initiate iron core formation in ferritin molecules.

This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.

This part was primarily used to construct BBa_K1189020 in order to allow us to express and purify the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 391

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1189024 short</partinfo>
-<p>This light ferritin chain comes from humans. This part along with heavy ferritin (<partinfo>BBa_K1189025</partinfo>), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures.
+<p>This light ferritin chain comes from humans. This part along with heavy ferritin (<partinfo>BBa_K1189025</partinfo>), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is a robust, remaining stable at extreme pHs and temperatures. The light ferritin purportedly contributes to nucleation to initiate iron core formation in ferritin molecules.
 </p>
 <p>