Difference between revisions of "Part:BBa K1150005"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150005 short</partinfo>
 
<partinfo>BBa_K1150005 short</partinfo>
  
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PIF6 ...
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|PIF6
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|-
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|'''Function'''
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|Phytochrome interaction factor
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Organism'''
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|Arabidopsis thaliana
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|-
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|'''Source'''
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|AG Weber, Freiburg
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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The Phytochrome Interaction Factor 6 (PIF6) is derived from Arabidopsis thaliana.
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Illumination with red light (660 nm wavelength) leads to binding of Phytochrome Interaction Factor 6 (PIF6) to Phytochrome B. The interaction can be abolished by illumination with far-red light (740 nm) wavelength.
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A framework using the unique properties of PhyB and PIF6 was designed to enable the recruitment of different effector proteins to any desired gene locus. The system was designed to work as described: Fusion proteins linking dCas9 to PIF6 were designed, as well as fusion proteins linking the interaction partner of PIF6 – PhyB – to several effector molecules such as VP16 or KRAB.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1150005 SequenceAndFeatures</partinfo>
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Revision as of 09:02, 28 September 2013

PIF6


PIF6
Function Phytochrome interaction factor
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Arabidopsis thaliana
Source AG Weber, Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

The Phytochrome Interaction Factor 6 (PIF6) is derived from Arabidopsis thaliana.

Illumination with red light (660 nm wavelength) leads to binding of Phytochrome Interaction Factor 6 (PIF6) to Phytochrome B. The interaction can be abolished by illumination with far-red light (740 nm) wavelength.

A framework using the unique properties of PhyB and PIF6 was designed to enable the recruitment of different effector proteins to any desired gene locus. The system was designed to work as described: Fusion proteins linking dCas9 to PIF6 were designed, as well as fusion proteins linking the interaction partner of PIF6 – PhyB – to several effector molecules such as VP16 or KRAB.