Difference between revisions of "Part:BBa K1195007:Design"

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<partinfo>BBa_K1195007 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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A strong terminator was incorporated into the end of GFP.
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Soeing PCR was used to fuse the YdiV promoter (right up to the initiating ATG) to superfolder GFP.
  
 
===Source===
 
===Source===
  
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YdiV comes from E. coli. GFP is from pGLO.
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YdiV comes from E. coli. Superfolder GFP is from the iGEM registry.
  
 
===References===
 
===References===
 +
Zhou, Xianxuan, Xiaoming Meng, and Baolin Sun. "An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli." Cell research 18.9 (2008): 937-948.
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Wada, Takeo, et al. "EAL domain protein YdiV acts as an anti-FlhD4C2 factor responsible for nutritional control of the flagellar regulon in Salmonella enterica Serovar Typhimurium." Journal of bacteriology 193.7 (2011): 1600-1611.

Latest revision as of 06:22, 28 September 2013

promoter ydiV + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Soeing PCR was used to fuse the YdiV promoter (right up to the initiating ATG) to superfolder GFP.

Source

YdiV comes from E. coli. Superfolder GFP is from the iGEM registry.

References

Zhou, Xianxuan, Xiaoming Meng, and Baolin Sun. "An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli." Cell research 18.9 (2008): 937-948.

Wada, Takeo, et al. "EAL domain protein YdiV acts as an anti-FlhD4C2 factor responsible for nutritional control of the flagellar regulon in Salmonella enterica Serovar Typhimurium." Journal of bacteriology 193.7 (2011): 1600-1611.