Difference between revisions of "Part:BBa K1129012"
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This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts. | This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts. | ||
+ | |||
+ | This part is computationally verified to target all of 13a, K30, T3 and T7 genomes | ||
Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. | Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. |
Revision as of 04:41, 28 September 2013
T7 Repeat/Spacer Region of T7 Bacteriophage
T7 Repeat/Spacer Region of Streptococcus thermophilus
This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts.
This part is computationally verified to target all of 13a, K30, T3 and T7 genomes
Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]