Difference between revisions of "Part:BBa K1166000"
MArellanoMtz (Talk | contribs) |
MArellanoMtz (Talk | contribs) |
||
| Line 6: | Line 6: | ||
==Characterization== | ==Characterization== | ||
| − | <p>After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. | + | <p>After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. |
[[File:HIP-1 characterization.jpg|800 px|]] | [[File:HIP-1 characterization.jpg|800 px|]] | ||
Latest revision as of 03:42, 28 September 2013
FNR-HIP1
HIP-1 drives gene expression under both acute and chronic hypoxia, but not under normoxia (Mengesha, et al., 2006). This part is composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.
Characterization
After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate.
The graphic shows that promoter HIP-1 may not be positively regulated by hypoxia, in fact, the data suggests that it may be negatively regulated by this condition.
References
Mengesha A, Dubois L, Lambin P, Landuyt W, Chiu RK, Wouters BG, Theys J. (2006). Development of a flexible and potent hypoxia-inducible promoter for tumor-targeted gene expression in attenuated Salmonella. Cancer Biol Ther. 5(9):1120-8.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]