Difference between revisions of "Part:BBa K1021020:Experience"
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===Applications of BBa_K1021020=== | ===Applications of BBa_K1021020=== | ||
| + | This construct was modified to contain a bacterial RBS so that we could characterize its transcriptional functionality. A fluorescence assay was run using a 96 well plate as described in the protocol section. T7+RBS+GFP and T7+GFP (BBa_K1021020) constructs in <i>E. coli</i> BL21-A1 were examined for their relative fluorescence (fluorescence/OD600). A T7 construct in <i>E. coli</i> BL21-A1 without downstream elements was utilized as a control along with the use of the aforementioned constructs in <i>E. coli</i> DH5α. As can be seen from the graph there were evident differences in peak fluorescence between the T7+RBS+GFP construct and the T7+GFP construct and controls. The T7+GFP+RBS construct appears to have a 20 fold higher relative fluorescence than the T7+GFP construct or the controls. | ||
| + | <br> | ||
| + | [[File:Fluorreal.png]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:42, 28 September 2013
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1021020
This construct was modified to contain a bacterial RBS so that we could characterize its transcriptional functionality. A fluorescence assay was run using a 96 well plate as described in the protocol section. T7+RBS+GFP and T7+GFP (BBa_K1021020) constructs in E. coli BL21-A1 were examined for their relative fluorescence (fluorescence/OD600). A T7 construct in E. coli BL21-A1 without downstream elements was utilized as a control along with the use of the aforementioned constructs in E. coli DH5α. As can be seen from the graph there were evident differences in peak fluorescence between the T7+RBS+GFP construct and the T7+GFP construct and controls. The T7+GFP+RBS construct appears to have a 20 fold higher relative fluorescence than the T7+GFP construct or the controls.
User Reviews
UNIQ9246f45e17016583-partinfo-00000000-QINU UNIQ9246f45e17016583-partinfo-00000001-QINU