Difference between revisions of "Part:BBa K1166000"
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<p>After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. Two readings were done, adjusting the amount of total protein in each well to 250ng and 500ng for each sample.</p> | <p>After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. Two readings were done, adjusting the amount of total protein in each well to 250ng and 500ng for each sample.</p> | ||
− | [[File:HIP-1 characterization.jpg]] | + | [[File:HIP-1 characterization.jpg|800|]] |
==References== | ==References== |
Revision as of 03:33, 28 September 2013
FNR-HIP1
HIP-1 drives gene expression under both acute and chronic hypoxia, but not under normoxia (Mengesha, et al., 2006). This part is composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.
Characterization
After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. Two readings were done, adjusting the amount of total protein in each well to 250ng and 500ng for each sample.
References
Mengesha A, Dubois L, Lambin P, Landuyt W, Chiu RK, Wouters BG, Theys J. (2006). Development of a flexible and potent hypoxia-inducible promoter for tumor-targeted gene expression in attenuated Salmonella. Cancer Biol Ther. 5(9):1120-8.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]