Difference between revisions of "Part:BBa K1139019"

Latest revision as of 03:32, 28 September 2013

Promoterless-M13 on pSB3

Fig. 1. M13 family

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter upstream of g2p is deleted. The capsid is formed only when g2p is expressed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 99
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6027
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1139019 short</partinfo>
+[[Image:Titech2013_m13_Fig_2-2-6.png|thumb|center|700px|<b>Fig. 1.</b> M13 family]]
 M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
-This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter on the upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br>
+This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br>
 <!-- Add more about the biology of this part here