Difference between revisions of "Part:BBa K1139019"
 
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<partinfo>BBa_K1139019 short</partinfo>
 
<partinfo>BBa_K1139019 short</partinfo>
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[[Image:Titech2013_m13_Fig_2-2-6.png|thumb|center|700px|<b>Fig. 1.</b> M13 family]]
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M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
 
M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
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This part is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter on the upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br>
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This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:32, 28 September 2013

Promoterless-M13 on pSB3

Fig. 1. M13 family


M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter upstream of g2p is deleted. The capsid is formed only when g2p is expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6027
  • 1000
    COMPATIBLE WITH RFC[1000]