Difference between revisions of "Part:BBa K1129028"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1129028 short</partinfo>
 
<partinfo>BBa_K1129028 short</partinfo>
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Arab+sense tracRNA+term
 
Arab+sense tracRNA+term
  
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There are several pathways of CRISPR activation, one of which requires a tracrRNA which plays a role in the maturation of crRNA. TracrRNA is complementary to and base pairs with a pre-crRNA forming an RNA duplex.
===Usage and Biology===
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http://en.wikipedia.org/wiki/TracrRNA
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1129028 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1129028 SequenceAndFeatures</partinfo>

Latest revision as of 03:30, 28 September 2013

antisense tracRNA under arabinose promoter

Arab+sense tracRNA+term

There are several pathways of CRISPR activation, one of which requires a tracrRNA which plays a role in the maturation of crRNA. TracrRNA is complementary to and base pairs with a pre-crRNA forming an RNA duplex. http://en.wikipedia.org/wiki/TracrRNA Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]