Difference between revisions of "Part:BBa K1230004"
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This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator (Part BBa_J04500) | This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator (Part BBa_J04500) | ||
| − | + | <img src="https://static.igem.org/mediawiki/igem.org/8/8b/U102_Fig2.png" width="1" height="1"/> | |
| + | <img src="https://static.igem.org/mediawiki/igem.org/2/21/U101_Fig3.png" width="1" height="1"/> | ||
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Revision as of 03:28, 28 September 2013
MarA Generator
The MarA protein acts as a transcriptional activator in E. coli. It allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.
This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator (Part BBa_J04500)
<img src="
" width="1" height="1"/>
<img src="
" width="1" height="1"/>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 380
- 1000COMPATIBLE WITH RFC[1000]