Difference between revisions of "Part:BBa K1019000"

 
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<partinfo>BBa_K1019000 short</partinfo>
 
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"FimB, along with FimE, is a recombinase in Escherichia coli which catalyzes the site-specific recombination required for inversion of a 314-bp invertible DNA element, the fim switch (fimS), to control transcription of the type I fimbrial structural genes--a process known as phase-variation switching. fimS contains the promoter for expression of the fimbrial structural genes. The promoter is positioned to allow expression of fimAICDFGH when fimS is in the ON orientation, but not when it is in the OFF orientation. FimB is responsible for both OFF-to-ON and ON-to-OFF switching at equal rates. FimE is responsible for primarily ON-to-OFF switching." (http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309)
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The fimB gene encodes for the FimB protein, a transcriptional regulator which mediates '''off to on''' switching of the major type 1 subunit fimbrin (pilin) FimA and other fimbrial genes in the fim operon.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The fimE gene acts opposite to fimB by mediating the '''on to off''' switching of the fim switch/promoter, fimS, which allows fimA (and associated fim operon genes) to be expressed depending on its state. Both fimB and fimE are recombinases that catalyze the inversion of fimS, thus controlling the expression of fimbriae.
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<span class='h3bb'>Sequence and Features</span>
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Overexpression of fimB should in principle encourage fimbriae formation, thus leading to increased adherence to cells and surfaces. Fimbriae expression constitutes a defining feature of biofilms.
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For further information please refer to [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309 EcoCyc].
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===References===
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Escherichia coli K-12 substr. MG1655 Polypeptide: regulator for fimA. (2013). Ecocyc. Retrieved May 26, 2013, from http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K1019000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1019000 SequenceAndFeatures</partinfo>
  

Latest revision as of 03:25, 28 September 2013

FimB: positive regulator of type I fimbriae

The fimB gene encodes for the FimB protein, a transcriptional regulator which mediates off to on switching of the major type 1 subunit fimbrin (pilin) FimA and other fimbrial genes in the fim operon.

Usage and Biology

The fimE gene acts opposite to fimB by mediating the on to off switching of the fim switch/promoter, fimS, which allows fimA (and associated fim operon genes) to be expressed depending on its state. Both fimB and fimE are recombinases that catalyze the inversion of fimS, thus controlling the expression of fimbriae.

Overexpression of fimB should in principle encourage fimbriae formation, thus leading to increased adherence to cells and surfaces. Fimbriae expression constitutes a defining feature of biofilms.

For further information please refer to [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309 EcoCyc].

References

Escherichia coli K-12 substr. MG1655 Polypeptide: regulator for fimA. (2013). Ecocyc. Retrieved May 26, 2013, from http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]