Difference between revisions of "Part:BBa K1129037"
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The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.  
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The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.  
  
 
'''Resources'''
 
'''Resources'''

Revision as of 03:09, 28 September 2013

4-coumarate-CoA ligase (4CL) under arabinose promoter

Arab.+rbs+4-CL+term.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1809
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1441



The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.

Resources

http://www.uniprot.org/uniprot/Q1H8P3

http://en.wikipedia.org/wiki/4-Coumarate-CoA_ligase

http://www.genome.jp/kegg-bin/show_pathway?map00940