Difference between revisions of "Part:BBa K1162006"

Revision as of 02:20, 28 September 2013

LL-37 antimicrobial peptide from Humans (Homo sapiens)

LL-37 is an antimicrobial peptide (AMP) found in humans.

Design

This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.

Improvement on Existing Parts (as of September 2013)

BBa_K245114 (Slovenia iGEM team 2009) - This part lacks a start codon. The BBa_K1162006 part improves upon this one by adding a Met(atg) residue at the beginning of the codon region. It is unclear if Slovenia's part has been codon optimized for E. coli, but our new part has been by Life Technologies (see design).

BBa_K875009 (Trieste iGEM team 2012) - This part, BBa_K875009, has a BioBrick RFC 10 prefix and suffix and therefore cannot be used for protein fusions, such as in cases of secretion, protein purification, or fusion proteins. This part also has a stop codon which further limits what can be added to the C-terminal end (purification tag/secretion tag). The BBa_K1162006 improves upon this part by using Assembly Standard #23 (Silver Fusion) and by removing the stop codon at the end of the coding region. Additionally, the BBa_K1162006 part was codon optimized by Life Technologies (see design).

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Improvement on Existing Parts (as of September 2013)===
-[https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114] Slovenia iGEM team 2009 - lacks a start codon.
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114] (Slovenia iGEM team 2009) - This part lacks a start codon. The BBa_K1162006 part improves upon this one by adding a Met(atg) residue at the beginning of the codon region. It is unclear if Slovenia's part has been codon optimized for <i>E. coli</i>, but our new part has been by Life Technologies (see design).
-Previously this part has been used Trieste iGEM team 2012
-https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009
+[https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 BBa_K875009] (Trieste iGEM team 2012) - This part, BBa_K875009, has a BioBrick RFC 10 prefix and suffix and therefore cannot be used for protein fusions, such as in cases of secretion, protein purification, or fusion proteins. This part also has a stop codon which further limits what can be added to the C-terminal end (purification tag/secretion tag). The BBa_K1162006 improves upon this part by using [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and by removing the stop codon at the end of the coding region. Additionally, the BBa_K1162006 part was codon optimized by Life Technologies (see design).
-This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification.  Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag).
 ===References===