Difference between revisions of "Part:BBa K1092003:Experience"
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The PCR product meets the target size (936bp), the DNA sequence was further verified by DNA sequencing. | The PCR product meets the target size (936bp), the DNA sequence was further verified by DNA sequencing. | ||
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2. Time series degradation assay | 2. Time series degradation assay | ||
| + | Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem ''et al''., 2011). | ||
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Time series analysis were performed. According to a chi-square test (Rosenberg ''et al''., 1998), the trends of hydroquinone degradation rate in E. coli BL21 transformed with catechol 1,2-dioxygenase and that in wild type E. coli BL21 were very highly statistically different (p < 0.001) (Figure 1). | Time series analysis were performed. According to a chi-square test (Rosenberg ''et al''., 1998), the trends of hydroquinone degradation rate in E. coli BL21 transformed with catechol 1,2-dioxygenase and that in wild type E. coli BL21 were very highly statistically different (p < 0.001) (Figure 1). | ||
Revision as of 02:07, 28 September 2013
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Characterization of BBa_K1092003
1. Biobrick DNA length verification
To check the inserted biobrick DNA length, PCR was conducted.
The PCR product meets the target size (936bp), the DNA sequence was further verified by DNA sequencing.
2. Time series degradation assay
Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem et al., 2011).
Time series analysis were performed. According to a chi-square test (Rosenberg et al., 1998), the trends of hydroquinone degradation rate in E. coli BL21 transformed with catechol 1,2-dioxygenase and that in wild type E. coli BL21 were very highly statistically different (p < 0.001) (Figure 1).
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