Difference between revisions of "Part:BBa K1011000"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1011000 short</partinfo> | <partinfo>BBa_K1011000 short</partinfo> | ||
| − | This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli | + | This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli minicells. |
| − | <!-- | + | As this picture illustrates, under normal WT conditions, FtsZ will form the Z ring at the middle of the cell. When overexpressed, FtsZ will polymerize non specifically at the polar ends of the cell, thereby cleaving the cell to form a minicell. |
| + | |||
| + | [[File:FtsZ Comparison.png]] | ||
| + | |||
| + | <!-- As this picture illustrates, under normal WT conditions, FtsZ will form the Z ring at the middle of the cell. When overexpressed, FtsZ will polymerize non specifically at the polar ends of the cell, thereby cleaving the cell to form a minicell. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | |||
<!-- --> | <!-- --> | ||
Revision as of 01:53, 28 September 2013
ftsZ gene from E. coli on pSB1C3
This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli minicells.
As this picture illustrates, under normal WT conditions, FtsZ will form the Z ring at the middle of the cell. When overexpressed, FtsZ will polymerize non specifically at the polar ends of the cell, thereby cleaving the cell to form a minicell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 322
- 1000COMPATIBLE WITH RFC[1000]