Difference between revisions of "Part:BBa K1129006:Experience"

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The Cas9 construct was transformed into <i>E. cloni</i> 10G, plated on LB agar plates made with chloramphenicol (33ug/mL), and grown overnight at 37C.
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A single colony was picked and grown overnight in LB (with chloramphenicol) at 37C and shaking (1000 rpm). Growth was very slow for the  and OD was only ar
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<img src="https://static.igem.org/mediawiki/igem.org/7/72/UBCCas9gel.png" width="300"> </img>
 
<img src="https://static.igem.org/mediawiki/igem.org/7/72/UBCCas9gel.png" width="300"> </img>

Revision as of 01:01, 28 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1129006

The Cas9 construct was transformed into E. cloni 10G, plated on LB agar plates made with chloramphenicol (33ug/mL), and grown overnight at 37C. A single colony was picked and grown overnight in LB (with chloramphenicol) at 37C and shaking (1000 rpm). Growth was very slow for the and OD was only ar


[File:UBC-Growth_curves_MOI.png|800px]

User Reviews

UNIQ968a48bbe3638cd2-partinfo-00000001-QINU UNIQ968a48bbe3638cd2-partinfo-00000002-QINU