Difference between revisions of "Part:BBa K1051204"
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<h3>Engineering</h3>
 
<h3>Engineering</h3>
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We convert base "A" to "G" at 24 site of cln3-PEST by designing primer.
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We convert base "A" to "G" at 6 site of cln3-PEST by designing primer.
  
 
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Revision as of 00:59, 28 September 2013

cln3-PEST

Purpose

Works for the degradation of fluorescent proteins we used at C-terminal.

Principle

PEST,Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.

Engineering

We convert base "A" to "G" at 6 site of cln3-PEST by designing primer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975.
[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".