Difference between revisions of "Part:BBa K1162001"
 
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<partinfo>BBa_K1162001 short</partinfo>
 
<partinfo>BBa_K1162001 short</partinfo>
  
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EcAMP-1 is derived from the grass species <i>Echinochloa crus-galli</i>. It consists of 37 amino acids and is helical in structure, held together by two disulfide bonds. This feature enables a unique hairpin structure which is not found in most other AMPs. EcAMP-1 has shown to have a toxic affect against a variety of fungi and protists. The mode of action for EcAMP-1 against fungi appears to be the prevention of hyphae elongation (Nolde et al., 2011). RFC 23 compatible. Contains ATG (methionine).
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EcAMP-1 is derived from the grass species <i>Echinochloa crus-galli</i>. It consists of 37 amino acids and is helical in structure, due in part to two disulfide bonds. This feature enables a unique hairpin structure which is not found in most other AMPs. EcAMP-1 has shown to have a toxic affect against a variety of fungi and protists. The mode of action for EcAMP-1 against fungi appears to be the prevention of hyphae elongation.
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===Design===
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===Usage and Biology===
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This part was codon optimized for <i>E. coli</i> using the Life Technologies GeneArt &#174; software program. By design, this part is [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and contains a Methionine(atg) residue at the start of the coding region.
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===References===
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Nolde, S., Vassilevski, A., Rogozhin, E., Barinov, N., Balashova, T., Samsonova, O., Baranov, Y., Feofanov, A., Egorov, A., Arseniev, A., Grishin, E., Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli). The Journal of Biological Chemistry. 2011. 286: 25145-25153.
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===Experience and Results===
  
 
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Latest revision as of 00:46, 28 September 2013

EcAMP-1 antimicrobial peptide from barnyard grass (Echinochloa crus-galli)

EcAMP-1 is derived from the grass species Echinochloa crus-galli. It consists of 37 amino acids and is helical in structure, due in part to two disulfide bonds. This feature enables a unique hairpin structure which is not found in most other AMPs. EcAMP-1 has shown to have a toxic affect against a variety of fungi and protists. The mode of action for EcAMP-1 against fungi appears to be the prevention of hyphae elongation.

Design

This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region.

References

Nolde, S., Vassilevski, A., Rogozhin, E., Barinov, N., Balashova, T., Samsonova, O., Baranov, Y., Feofanov, A., Egorov, A., Arseniev, A., Grishin, E., Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli). The Journal of Biological Chemistry. 2011. 286: 25145-25153.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]