Difference between revisions of "Part:BBa K1162001"
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<partinfo>BBa_K1162001 short</partinfo> | <partinfo>BBa_K1162001 short</partinfo> | ||
| − | + | EcAMP-1 is derived from the grass species <i>Echinochloa crus-galli</i>. It consists of 37 amino acids and is helical in structure, due in part to two disulfide bonds. This feature enables a unique hairpin structure which is not found in most other AMPs. EcAMP-1 has shown to have a toxic affect against a variety of fungi and protists. The mode of action for EcAMP-1 against fungi appears to be the prevention of hyphae elongation. | |
| + | <br> | ||
| + | |||
| + | ===Design=== | ||
| + | |||
| + | This part was codon optimized for <i>E. coli</i> using the Life Technologies GeneArt ® software program. By design, this part is [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and contains a Methionine(atg) residue at the start of the coding region. | ||
| + | |||
| + | ===References=== | ||
| + | Nolde, S., Vassilevski, A., Rogozhin, E., Barinov, N., Balashova, T., Samsonova, O., Baranov, Y., Feofanov, A., Egorov, A., Arseniev, A., Grishin, E., Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli). The Journal of Biological Chemistry. 2011. 286: 25145-25153. | ||
| − | <!-- | + | <!-- |
| − | === | + | ===Experience and Results=== |
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Latest revision as of 00:46, 28 September 2013
EcAMP-1 antimicrobial peptide from barnyard grass (Echinochloa crus-galli)
EcAMP-1 is derived from the grass species Echinochloa crus-galli. It consists of 37 amino acids and is helical in structure, due in part to two disulfide bonds. This feature enables a unique hairpin structure which is not found in most other AMPs. EcAMP-1 has shown to have a toxic affect against a variety of fungi and protists. The mode of action for EcAMP-1 against fungi appears to be the prevention of hyphae elongation.
Design
This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region.
References
Nolde, S., Vassilevski, A., Rogozhin, E., Barinov, N., Balashova, T., Samsonova, O., Baranov, Y., Feofanov, A., Egorov, A., Arseniev, A., Grishin, E., Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli). The Journal of Biological Chemistry. 2011. 286: 25145-25153.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]