Difference between revisions of "Part:BBa K1100105:Design"

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===Design Notes===
 
===Design Notes===
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coming soon
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To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry.
  
  

Latest revision as of 00:01, 28 September 2013

J23105 with insulator and sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry.


Source

J23105 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.