Difference between revisions of "Part:BBa K1100100"

Latest revision as of 00:00, 28 September 2013

J23100 with insulator and sfGFP

We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter J23100.

Figure 1. The distribution of the sfGFP expression tested by flow cytometry with promoter J23100 and Csy4 insulator.

Figure 2. The intensity of different promoters is shown by the column height and the randomness of their functions by the bar width.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1100100 short</partinfo>
-We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR.
+We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter J23100.
+[[File:pSB1C3-J23100-f.jpg|500px|thumb|center|'''Figure 1'''. The distribution of the sfGFP expression tested by flow cytometry with promoter J23100 and Csy4 insulator.]]
+[[File:fc--c.jpg|400px|thumb|center|'''Figure 2'''. The intensity of different promoters is shown by the column height and the randomness of their functions by the bar width.]]
 <!-- Add more about the biology of this part here