Difference between revisions of "Part:BBa K1100010:Design"
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<partinfo>BBa_K1100010 short</partinfo> | <partinfo>BBa_K1100010 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
To generate Csy4 protein | To generate Csy4 protein | ||
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===Source=== | ===Source=== | ||
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B0034 via PCR, | B0034 via PCR, | ||
Insulator: Csy4 loci via PCR, | Insulator: Csy4 loci via PCR, | ||
| − | + | B0015 from Kit. | |
===References=== | ===References=== | ||
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358. | Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358. | ||
Latest revision as of 23:56, 27 September 2013
Csy4 generator (with terminator)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 448
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 164
Design Notes
To generate Csy4 protein
Source
The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.