Difference between revisions of "Part:BBa K1100015:Design"

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<partinfo>BBa_K1100015 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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coming soon
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To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry.
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[[File:The plasmid map of K1100015.png]]
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===Source===
 
===Source===
  
 
R0010 from Kit,
 
R0010 from Kit,
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Csy4 loci via PCR,
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Insulator: Csy4 loci via PCR,
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sfGFP from Ding Yu lab,
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sfGFP from Ding Yu lab at Fudan University
  
 
===References===
 
===References===
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Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006;
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Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.
 
Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.
 
Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.

Latest revision as of 23:53, 27 September 2013

R0010 with insulator and sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry. The plasmid map of K1100015.png

Source

R0010 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.