Difference between revisions of "Part:BBa K1100015:Design"
(→References) |
(→Design Notes) |
||
| (6 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1100015 short</partinfo> | <partinfo>BBa_K1100015 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry. | |
| − | + | [[File:The plasmid map of K1100015.png]] | |
| − | + | ||
===Source=== | ===Source=== | ||
R0010 from Kit, | R0010 from Kit, | ||
| − | Csy4 loci via PCR, | + | Insulator: Csy4 loci via PCR, |
| − | sfGFP from Ding Yu lab | + | sfGFP from Ding Yu lab at Fudan University |
===References=== | ===References=== | ||
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. | Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. | ||
Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358. | Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358. | ||
Latest revision as of 23:53, 27 September 2013
R0010 with insulator and sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry.
Source
R0010 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.