Difference between revisions of "Part:BBa K1100014:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
coming soon
+
[[File:Plasmid1.jpg]]
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+
−
 
+
  
 
===Source===
 
===Source===
−
insulator is obtained via PCR;
+
Insulator: Csy4 loci via PCR,
−
ALeaderT is obtained via de novo gene synthesis
+
ALeaderT via de novo gene synthesis
  
 
===References===
 
===References===
  
 
Jia X, Zhang J, Sun W, et al. Riboswitch Control of Aminoglycoside Antibiotic Resistance[J]. Cell, 2013, 152(1): 68-81.
 
Jia X, Zhang J, Sun W, et al. Riboswitch Control of Aminoglycoside Antibiotic Resistance[J]. Cell, 2013, 152(1): 68-81.
 +
 +
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.
 +
 +
Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.

Latest revision as of 23:42, 27 September 2013


ALeaderT (with insulator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Plasmid1.jpg

Source

Insulator: Csy4 loci via PCR, ALeaderT via de novo gene synthesis

References

Jia X, Zhang J, Sun W, et al. Riboswitch Control of Aminoglycoside Antibiotic Resistance[J]. Cell, 2013, 152(1): 68-81.

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.

Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.