Difference between revisions of "Part:BBa K1189019:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1189019=== | ===Applications of BBa_K1189019=== | ||
| + | This protein was successfully expressed and purified through the use of his-tags. WESTERN BLOT STUFF | ||
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| + | We then modified the iron core in order to convert the protein into a peroxidase-like catalyst. Initial colourimetric testing was conducted and it was shown to be effective as the catalyst. PICTURE Qualitative kinetics testing was also conducted for commercial horsespleen ferritin to determine pH and temperature optimums, as well as Michaelis-Menten kinetics for the TMB substrates. PICTURESSSSSs | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 23:25, 27 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1189019
This protein was successfully expressed and purified through the use of his-tags. WESTERN BLOT STUFF
We then modified the iron core in order to convert the protein into a peroxidase-like catalyst. Initial colourimetric testing was conducted and it was shown to be effective as the catalyst. PICTURE Qualitative kinetics testing was also conducted for commercial horsespleen ferritin to determine pH and temperature optimums, as well as Michaelis-Menten kinetics for the TMB substrates. PICTURESSSSSs
User Reviews
UNIQa59d4a62e35c5bc0-partinfo-00000000-QINU UNIQa59d4a62e35c5bc0-partinfo-00000001-QINU