Difference between revisions of "Part:BBa K1114205"

Latest revision as of 23:19, 27 September 2013

CviI_CD

This is a MoClo formatted CviI gene with CD fusion sites. This is a LuxI-homologue. CviI is the inducer gene in quorum sensing in Chromobacterium violaceum.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Source

Chromobacterium violaceum strain ATCC 12472 genomic DNA, obtained from the Holden Lab at the University of Wisconsin-Madison

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 116
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 116
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 116
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 116
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1114205 short</partinfo>
-CviI of CviI/CviR system converted into Moclo for E. Coli
+<html>
+This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted CviI gene with CD fusion sites. This is a LuxI-homologue. CviI is the inducer gene in quorum sensing in <i>Chromobacterium violaceum</i>.
+<br><br>
+This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
+The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
+<br><br></html>
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 16: @@
 <!-- -->
+===Source===
+Chromobacterium violaceum strain ATCC 12472 genomic DNA, obtained from the Holden Lab at the University of Wisconsin-Madison
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1114205 SequenceAndFeatures</partinfo>