Difference between revisions of "Part:BBa K1036005"
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This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene. | This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene. | ||
Superfolder GFP with a LAV-tag is introduced in Part:<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a>, and quorum sensing mechanism is described in Part:<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036003">BBa_K1036003</a>. | Superfolder GFP with a LAV-tag is introduced in Part:<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a>, and quorum sensing mechanism is described in Part:<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036003">BBa_K1036003</a>. | ||
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=Experimental Data= | =Experimental Data= | ||
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| + | Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036005">BBa_K1036005</a> which was without lux promoter and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036006">BBa_K1036006</a> which was under lux promoter control.<br/><br/> | ||
<img src="https://static.igem.org/mediawiki/2013/d/d3/BBa_K1036005-Fig.1.png" width=500px; height=280px;/><br/><br/> | <img src="https://static.igem.org/mediawiki/2013/d/d3/BBa_K1036005-Fig.1.png" width=500px; height=280px;/><br/><br/> | ||
<img src="https://static.igem.org/mediawiki/2013/1/13/BBa_K1036005-Fig.2.png" width=250px; height=250px;/><br/><br/> | <img src="https://static.igem.org/mediawiki/2013/1/13/BBa_K1036005-Fig.2.png" width=250px; height=250px;/><br/><br/> | ||
Latest revision as of 23:15, 27 September 2013
sfgfp (with LVA-tag) under plux R control
Description
This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene. Superfolder GFP with a LAV-tag is introduced in Part:BBa_K1036004, and quorum sensing mechanism is described in Part:BBa_K1036003.
Experimental Data
Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of BBa_K1036005 which was without lux promoter and BBa_K1036006 which was under lux promoter control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2737
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914
Illegal BsaI.rc site found at 2017
Illegal SapI.rc site found at 31