Difference between revisions of "Part:BBa K1036005"
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<partinfo>BBa_K1036005 short</partinfo>
 
<partinfo>BBa_K1036005 short</partinfo>
  
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sfGFP+12D
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=Description=
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This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene.
 
This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene.
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Superfolder GFP with a LAV-tag is introduced in Part:BBa_K1036004, and quorum sensing mechanism is described in Part:BBa_K1036003.
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Superfolder GFP with a LAV-tag is introduced in Part:<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a>, and quorum sensing mechanism is described in Part:<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036003">BBa_K1036003</a>.
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=Experimental Data=
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Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036005">BBa_K1036005</a> which was without lux promoter and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036006">BBa_K1036006</a> which was under lux promoter control.<br/><br/>
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<img src="https://static.igem.org/mediawiki/2013/d/d3/BBa_K1036005-Fig.1.png" width=500px; height=280px;/><br/><br/>
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<img src="https://static.igem.org/mediawiki/2013/1/13/BBa_K1036005-Fig.2.png" width=250px; height=250px;/><br/><br/>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:15, 27 September 2013

sfgfp (with LVA-tag) under plux R control

Description

This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene. Superfolder GFP with a LAV-tag is introduced in Part:BBa_K1036004, and quorum sensing mechanism is described in Part:BBa_K1036003.

Experimental Data

Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of BBa_K1036005 which was without lux promoter and BBa_K1036006 which was under lux promoter control.






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914
    Illegal BsaI.rc site found at 2017
    Illegal SapI.rc site found at 31