Difference between revisions of "Part:BBa K1020011"
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| + | We put the resistance gene encoding tetA under the regulation of PalkM. When PalkM-RNAP complex isomerizes due to the interaction between alkanes and alkR, downstream TetA gene will express. Then, TetA will transport tetracycline out of the cell so that the intracellular concentration of tetracycline is relatively stable. and the growth of cells will not be inhibited. Thus, we can turn alkane molecules into the output of the growth rate of cells. | ||
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| + | <img src=" https://static.igem.org/mediawiki/igem.org/a/aa/QQ%E5%9B%BE%E7%89%8720130928070602.jpg" width="700px"/> | ||
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| + | We add alkanes outside the cell to test the function of TetA: | ||
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| + | We got a 3×3 matrix, with promoter strength from weak, medium to strong, and plasmid copy number from small, medium to large. | ||
Latest revision as of 23:12, 27 September 2013
High expressed Alkane Selector for intracellular alkane production
We put the resistance gene encoding tetA under the regulation of PalkM. When PalkM-RNAP complex isomerizes due to the interaction between alkanes and alkR, downstream TetA gene will express. Then, TetA will transport tetracycline out of the cell so that the intracellular concentration of tetracycline is relatively stable. and the growth of cells will not be inhibited. Thus, we can turn alkane molecules into the output of the growth rate of cells.
We add alkanes outside the cell to test the function of TetA:
We got a 3×3 matrix, with promoter strength from weak, medium to strong, and plasmid copy number from small, medium to large.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 346
Illegal NheI site found at 1544
Illegal NheI site found at 1567 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 492
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 518
Illegal NgoMIV site found at 886
Illegal NgoMIV site found at 1046 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2337