Difference between revisions of "Part:BBa K1189004:Design"
Sharonfeng1 (Talk | contribs) |
Sharonfeng1 (Talk | contribs) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | The target sequence was amplified with primers from the genome of <i>E. coli</i> using polymerase chain reaction. | |
===References=== | ===References=== | ||
Revision as of 22:48, 27 September 2013
TALEA Target ([A])
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 325 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone.
Source
The target sequence was amplified with primers from the genome of E. coli using polymerase chain reaction.