Difference between revisions of "Part:BBa K1100061"

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	<partinfo>BBa_K1100061 short</partinfo>		<partinfo>BBa_K1100061 short</partinfo>
		+	<p></p>It is the wild type RNA-OUT of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.
		+	It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
		+	[[File:RNA-IN-RNA-OUT.jpg|800px|thumb|center|Figure1. mechanism of RNA-INOUT regulatory system]]
		+	[[File:OUTS1.jpg|400px|thumb|center|Figure2. predicted structure of RNAOUTS1]]
		+	== References ==
		+	<p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.
−	It is the wild type RNA-OUT of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
−	[[File:Example.jpg]]
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
	===Usage and Biology===		===Usage and Biology===
		+
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Latest revision as of 22:13, 27 September 2013

RNA-OUT S1

It is the wild type RNA-OUT of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.

It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.

References

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features