Difference between revisions of "Part:BBa K1100013"
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<p></p>The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. | <p></p>The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. | ||
It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%. | It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%. | ||
| − | The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. | + | <p></p>The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. |
[[File:RNA-IN-RNA-OUT.jpg|800px|thumb|center|Figure1. mechanism of RNA-INOUT regulatory system]] | [[File:RNA-IN-RNA-OUT.jpg|800px|thumb|center|Figure1. mechanism of RNA-INOUT regulatory system]] | ||
[[File:OUTS1.jpg|400px|thumb|center|Figure2. predicted structure of RNAOUTS1]] | [[File:OUTS1.jpg|400px|thumb|center|Figure2. predicted structure of RNAOUTS1]] | ||
| − | References | + | == References == |
<p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454. | <p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454. | ||
Latest revision as of 22:12, 27 September 2013
R1051-OUT S1
The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.
References
Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]