Difference between revisions of "Part:BBa K1161000"

 
 
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Violacein A (Vio)A is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.
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Violacein A (Vio)A is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003.  
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:K1161000.jpeg|300px]]
  
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Features:
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1. Derived from the violacein operon constructed and submitted by Cambridge 2009 (BBa_K274002).
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2. Upgraded from pSB1K3 to pSB1C3 standard 10.
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3. Operon Orfs are now individual parts that can be assembled in different combinations and permutations.
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4. Each Orf shares a common and improved rbs for more rapid colour development.
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5. All have been characterized and are functional.
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6. The VioABCE combination produces a new pigment which we call “Royal Blue”
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1161000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1161000 SequenceAndFeatures</partinfo>

Latest revision as of 22:02, 27 September 2013

violacein A coding sequence

Violacein A (Vio)A is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003.

Usage and Biology

K1161000.jpeg

Features: 1. Derived from the violacein operon constructed and submitted by Cambridge 2009 (BBa_K274002). 2. Upgraded from pSB1K3 to pSB1C3 standard 10. 3. Operon Orfs are now individual parts that can be assembled in different combinations and permutations. 4. Each Orf shares a common and improved rbs for more rapid colour development. 5. All have been characterized and are functional. 6. The VioABCE combination produces a new pigment which we call “Royal Blue”

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1033
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]