Difference between revisions of "Part:BBa K1036004:Design"

(→‎Source)
(→‎Design Notes)
 
(7 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
add the LVA tag
+
<html>
−
 
+
<body>
−
 
+
The <i>sfgfp</i> gene with RBS (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</a>) was amplifed from the biobrick pSB1A3-RBS-sfgfp-TT provided by Peking Unversity via PCR. The LVA-tag and terminator(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>) were fused with <i>sfgfp</i> via fusion PCR. The DH5α colony carried <i>sfgfp</i> fusion gene was sequenced after TA-cloning.
 +
</body>
 +
</html>
  
 
===Source===
 
===Source===
 
<html>
 
<html>
 
<body>
 
<body>
−
The template of <i>sfgfp</i> gene namely biobrick pSB1C3-RBS-<i>sfgfp</i>-TT was provided by 2013 iGEM team <a href="http://2013.igem.org/Team:Peking">Peking</a>
+
The template of <i>sfgfp</i> gene namely biobrick pSB1C3-RBS-<i>sfgfp</i>-TT was provided by 2013 iGEM team <a href="http://2013.igem.org/Team:Peking">Peking</a>.We add the LVA-tag in the end of <i>sfgfp</i>.<br/>We thanks for Peking's help.
 
</body>
 
</body>
 
</html>
 
</html>
  
 
===References===
 
===References===
 +
<html>
 +
<body>
 +
1. <a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP">http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP</a><br/>
 +
2. <a href="http://www.ncbi.nlm.nih.gov/pubmed/16369541">Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)</a><br/>
 +
</body>
 +
</html>

Latest revision as of 21:52, 27 September 2013


sfgfp with LVA-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

The sfgfp gene with RBS (BBa_B0034) was amplifed from the biobrick pSB1A3-RBS-sfgfp-TT provided by Peking Unversity via PCR. The LVA-tag and terminator(BBa_B0015) were fused with sfgfp via fusion PCR. The DH5α colony carried sfgfp fusion gene was sequenced after TA-cloning.

Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)