Difference between revisions of "Part:BBa K1166001"

 
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<partinfo>BBa_K1166001 short</partinfo>
 
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The Fumarate-Nitrate Reductase protein or FNR helps hypoxic promoters to have a more sensitive hypoxic response, it works as an activator and repressor for oxygen-dependant genes. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
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FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008).
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Therefore co-expression of FNR when hypoxic promoters are used would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
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==References==
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Reinhart F, Achebach S, Koch T, Unden G. (2008). Reduced apo-fumarate nitrate reductase regulator (apoFNR) as the major form of FNR in aerobically growing Escherichia coli. J Bacteriol. 190(3):879-86.
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Revision as of 21:44, 27 September 2013

Fumarate-Nitrate Reductase

FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). Therefore co-expression of FNR when hypoxic promoters are used would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.

References

Reinhart F, Achebach S, Koch T, Unden G. (2008). Reduced apo-fumarate nitrate reductase regulator (apoFNR) as the major form of FNR in aerobically growing Escherichia coli. J Bacteriol. 190(3):879-86.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]