Difference between revisions of "Part:BBa K1054021"
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<partinfo>BBa_K1054021 short</partinfo> | <partinfo>BBa_K1054021 short</partinfo> | ||
− | + | This part is used to confirm the localization of membrane scaffold. Beta-lactamase is fused to the periplasmic end of membrane scaffold. If the fusion protein is successfully targeted to the inner-membrane, the bacteria will have ampicillin resistance. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 21:40, 27 September 2013
ssDsbA+beta-lactamase+lgt+streptavidin
This part is used to confirm the localization of membrane scaffold. Beta-lactamase is fused to the periplasmic end of membrane scaffold. If the fusion protein is successfully targeted to the inner-membrane, the bacteria will have ampicillin resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1862
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1664
Illegal AgeI site found at 1904
Illegal AgeI site found at 1955 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 723
Illegal BsaI site found at 1285