Difference between revisions of "Part:BBa K1031015"
 
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<partinfo>BBa_K1031015 short</partinfo>
 
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<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter</a></p>
  
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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cI protein,which is a transcription factor,is a part of bandpass filter.It can repress the hybrid promoter K1031021[https://parts.igem.org/Part:BBa_K1031021].In order to fine-tune the band-pass filter,we construct a libiary of rbs before cI protein.
 
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===Usage and Biology===
 
  
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== '''Components and Characterization''' ==
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== '''Sequence and Features''' ==
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K1031015 consists of <html><a href="https://parts.igem.org/Part:BBa_J61051">NahR</a></html>, Psal promoter, RBS <html><a href="https://parts.igem.org/Part:BBa_B0032">B0032</a></html> and transcription factor CƖ. CƖ functions as a repressor of <html><a href="https://parts.igem.org/Part:BBa_K1031021">hybrid promoter</a></html>. It is the repression branch of the Band-pass Filter circuit ('''Fig 1'''). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031015 adopts RBS B0032. 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/2/27/K1031015.png/800px-K1031015.png" style="width:750px; margin-left:100px"  />
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<p style="text-align:center"><b>Fig 1</b> Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating <i>Psal</i> promoter shown in light blue. Once <i>Psal</i> promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibiting hybrid promoter marked with two colors, thus inhibiting expression of sfGFP. The RBS B0032 is shown in light green, regulating the expression level of CƖ protein. 
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== '''Sequence and Features''' ==
 
<partinfo>BBa_K1031015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031015 SequenceAndFeatures</partinfo>
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Latest revision as of 21:15, 27 September 2013

NahR-Psal-B0032-RiboJ-CI

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter


Components and Characterization

K1031015 consists of NahR, Psal promoter, RBS B0032 and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter. It is the repression branch of the Band-pass Filter circuit (Fig 1). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031015 adopts RBS B0032.

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once Psal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibiting hybrid promoter marked with two colors, thus inhibiting expression of sfGFP. The RBS B0032 is shown in light green, regulating the expression level of CƖ protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]