Difference between revisions of "Part:BBa K741002:Experience"

Latest revision as of 20:58, 27 September 2013

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Applications of BBa_K741002

User Reviews

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No review score entered. 2013SUSTC-Shenzhen-A

[http://2013.igem.org/Team:SUSTC-Shenzhen-A 2013SUSTC-Shenzhen-A] used BBa_K741002 and got some data from experiment.

Seeing BBa_K741002’s figure prepared by USTC_China, we were thinking whether we could get it more efficient in decreasing the fluorescence produced by the bacteria. Besides, we though it lacked the data under the low concentration of glucose. So we measured this part by microplate in the concentration showing below. The result came out to be very good: in about 10-2.4M glucose, the fluorescence has left only a half in one cell.With the increasing of glucose, the fluorescence/OD continuously decreases, and at about 10-1M glucose, the fluorescence/OD remains very low.

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 <I>2013SUSTC-Shenzhen-A</I>
 |width='60%' valign='top'|
-SUSTC-Shenzhen-A[http://2013.igem.org/Team:SUSTC-Shenzhen-A]used BBa_K741002 and got some data from experiment.
+[http://2013.igem.org/Team:SUSTC-Shenzhen-A 2013SUSTC-Shenzhen-A] used BBa_K741002 and got some data from experiment.
-Compared with testing result from USTC_China, we’ve got a better data. According to the data from USTC_China, after adding glucose, the fluorescence reduces only about 10% in the most obvious one. However, we got a clear reduce in the production of fluorescence. In about 10-2.4M glucose, the fluorescence has left only a half in one cell. With the increasing of glucose, the fluorescence/OD continuously decreases, and at about 10-1M glucose, the fluorescence/OD remains very low.
+Seeing BBa_K741002’s figure prepared by USTC_China, we were thinking whether we could get it more efficient in decreasing the fluorescence produced by the bacteria. Besides, we though it lacked the data under the low concentration of glucose. So we measured this part by microplate in the concentration showing below. The result came out to be very good: in about 10-2.4M glucose, the fluorescence has left only a half in one cell.With the increasing of glucose, the fluorescence/OD continuously decreases, and at about 10-1M glucose, the fluorescence/OD remains very low.
 [[File:741002-glucose.jpg]]
 |};
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