Difference between revisions of "Part:BBa K741002:Experience"

 
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<I>2013SUSTC-Shenzhen-A</I>
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[http://2013.igem.org/Team:SUSTC-Shenzhen-A 2013SUSTC-Shenzhen-A] used BBa_K741002 and got some data from experiment.
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Seeing BBa_K741002’s figure prepared by USTC_China, we were thinking whether we could get it more efficient in decreasing the fluorescence produced by the bacteria. Besides, we though it lacked the data under the low concentration of glucose. So we measured this part by microplate in the concentration showing below. The result came out to be very good: in about 10-2.4M glucose, the fluorescence has left only a half in one cell.With the increasing of glucose, the fluorescence/OD continuously decreases, and at about 10-1M glucose, the fluorescence/OD remains very low.
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[[File:741002-glucose.jpg]]
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Latest revision as of 20:58, 27 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K741002

User Reviews

UNIQc8389f51ed5f4cf6-partinfo-00000000-QINU

No review score entered. 2013SUSTC-Shenzhen-A

[http://2013.igem.org/Team:SUSTC-Shenzhen-A 2013SUSTC-Shenzhen-A] used BBa_K741002 and got some data from experiment.

Seeing BBa_K741002’s figure prepared by USTC_China, we were thinking whether we could get it more efficient in decreasing the fluorescence produced by the bacteria. Besides, we though it lacked the data under the low concentration of glucose. So we measured this part by microplate in the concentration showing below. The result came out to be very good: in about 10-2.4M glucose, the fluorescence has left only a half in one cell.With the increasing of glucose, the fluorescence/OD continuously decreases, and at about 10-1M glucose, the fluorescence/OD remains very low.

741002-glucose.jpg

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UNIQc8389f51ed5f4cf6-partinfo-00000002-QINU