Difference between revisions of "Part:BBa K1024005"

Latest revision as of 20:31, 27 September 2013

Report yeast (inducible ADE2)

Part Name: BBa_K1024005

Short Description: Report yeast (inducible ADE2)

Part Type: Signaling

Design: The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white.

Figure 1. The model of reporter yeast

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1053
Illegal BamHI site found at 456
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1024005 short</partinfo>
-[[File:https://static.igem.org/mediawiki/igem.org/a/a5/Tsinghua-BB-005.png]]
 <p>
 					<b>Part Name:</b> BBa_K1024005
@@ Line 15: / Line 15: @@
 					<b>Design:</b> The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white.
 				</p>
+https://static.igem.org/mediawiki/igem.org/a/a5/Tsinghua-BB-005.png
+<b>Figure 1. The model of reporter yeast </b><br>
 <!-- Add more about the biology of this part here