Difference between revisions of "Part:BBa K1024004"

Latest revision as of 20:30, 27 September 2013

Sensor yeast (inducible TetR+VP16)

Part Name: BBa_K1024004

Short Description: Sensor yeast (inducible TetR+VP16)

Part Type: Signaling

Design: The part was the sensor of the pathogen detector. LuxR was constitutively expressed. When sensing AHL, LuxR will bind to the Lux promoter and thus activated the downstream TetR with VP16. Mated with reporter yeast (BBa_K1024005), the TetR with VP16 will bind to the Tet operator and activated the downstream gene.

Figure 1. The model of sensor yeast
Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 2555
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1580
Illegal BamHI site found at 2546
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 2555
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 2555
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 506
Illegal BsaI site found at 893
Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 547
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1321

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1024004 short</partinfo>
-TF6
+<p>
+					<b>Part Name:</b> BBa_K1024004
+				</p>
+				<p>
+					<b>Short Description:</b> Sensor yeast (inducible TetR+VP16)
+				</p>
+				<p>
+					<b>Part Type:</b> Signaling
+				</p>
+				<p>
+					<b>Design:</b> The part was the sensor of the pathogen detector. LuxR was constitutively expressed. When sensing AHL, LuxR will bind to the Lux promoter and thus activated the downstream TetR with VP16. Mated with reporter yeast (BBa_K1024005), the TetR with VP16 will bind to the Tet operator and activated the downstream gene.
+				</p>
+https://static.igem.org/mediawiki/igem.org/a/a9/Tsinghua-BB-004.png
+<b>Figure 1. The model of sensor yeast </b><br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===