Difference between revisions of "Part:BBa K1124010:Design"

 
 
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<partinfo>BBa_K1124010 short</partinfo>
 
<partinfo>BBa_K1124010 short</partinfo>
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===Design Notes===
 
===Design Notes===
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PCR
 
  
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This part was generated by site-directed mutagenesis on MicC-sRNA scaffold([[BBa_K1124005]]), in which the target-binding sequence was added to the scaffold. The target binding sequence was designed complementary to the csrA gene of E. coli K-12 strain.
  
  
 
===Source===
 
===Source===
  
−
PCR
+
We designed oligo DNA complementary to the first 24nt of csrA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold ([[BBa_K1124005]]) using PCR technique.
  
 
===References===
 
===References===
 +
 +
Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.
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 +
Revelles, O., Millard, P., Nougayrède, J. P., Dobrindt, U., Oswald, E., Létisse, F., & Portais, J. C. (2013). The Carbon Storage Regulator (Csr) System Exerts a Nutrient-Specific Control over Central Metabolism in Escherichia coli Strain Nissle 1917. PloS one, 8(6), e66386.

Latest revision as of 20:18, 27 September 2013

anti-csrA sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was generated by site-directed mutagenesis on MicC-sRNA scaffold(BBa_K1124005), in which the target-binding sequence was added to the scaffold. The target binding sequence was designed complementary to the csrA gene of E. coli K-12 strain.


Source

We designed oligo DNA complementary to the first 24nt of csrA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold (BBa_K1124005) using PCR technique.

References

Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.

Revelles, O., Millard, P., Nougayrède, J. P., Dobrindt, U., Oswald, E., Létisse, F., & Portais, J. C. (2013). The Carbon Storage Regulator (Csr) System Exerts a Nutrient-Specific Control over Central Metabolism in Escherichia coli Strain Nissle 1917. PloS one, 8(6), e66386.